Molecular Biology Enzymes and Reagents

T3/SP6/T7 RNA Polymerase
DNA-Dependent RNA Polymerase Specific for T3/SP6/T7 Promoter Sequences
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

應用:

• Generation of templates for in vitro translation.
• Generation of probes for nucleic acid hybridizations.
• Generation of RNA processing substrates.
• Generation of antisense RNA

詳細介紹
 

T3 RNA Polymerase

• Exhibits extremely high affinity and specificity for T3 promoter sequences
• >90% pure as determined by SDS polyacrylamide gel electrophoresis
• Free of detectable levels of contaminating RNase and DNase activity (<1% release)
• Supplied at a concentration of 10–20u/μl (Cat.# P2083) and 80u/µl (Cat.# P4024)

SP6 RNA Polymerase

• Exhibits extremely high affinity and specificity for SP6 promoter sequences
• >90% pure as determined by SDS polyacrylamide gel electrophoresis
• Free of detectable levels of contaminating RNase and DNase activity (<1% release)

T7 RNA Polymerase

• >90% pure as determined by SDS polyacrylamide gel electrophoresis
• Free of detectable levels of contaminating RNase and DNase activity (<1% release)
• Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates