Bottoms Up: Improving Proteomic Sample Preparation with Recombinant Asp-N and...

Bottoms Up: Improving Proteomic Sample Preparation with Recombinant Asp-N and Multi-Enzyme Workflows

Tuesday, October 9, 2018

While trypsin is the workhorse for bottom-up proteomics, it is not a magic bullet since trypticdigestion often leaves gaps in protein sequences. In this webinar we will describe the advantages ofusing a new recombinant Asp-N protease to fill in the gaps. We will also demonstrate how combining digests from multiple proteases can yield comprehensive sequence coverage of proteins from a single LC-MS/MS run.

Chris Hosfield, PhD

Chris Hosfield is a Senior Research Scientist in the Protein Analysis group at Promega.

He has lead the development of tools for proteomics and antibody characterization, including PNGase F and IdeS and recombinant Asp-N proteases.Chris has broad expertise working with proteolytic enzymes and preparing protein samples for analysis by mass spectrometry. Prior to joining Promega, Chris held senior scientist positions at several biotechnology companies where he applied proteomic methods to the discovery and development of both small molecules and biotherapeutics. He received his PhD in biochemistry from Queen's University.