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Applications of a Bioluminescent Peptide Tag: Simple, Quantitative Protein Detection......
Applications of a Bioluminescent Peptide Tag: Simple, Quantitative Protein Detection Down to Endogenous Levels
Regulated protein abundance and surface expressionare fundamental to normal cellular physiology and dysregulation underlies many disease states. Monitoring changes in protein abundance usually involves labor-intensive, antibody-based methods that yield only semi-quantitative results. This webinar will introduce a bioluminescent tagging system that can quantify proteins in minutes using simple, antibody-free methods. This system offers wide dynamic range and sensitivity compatible with detecting even endogenously expressed proteins when combined with CRISPR gene editing.
Christopher Eggers, PhD
Sr Research Scientist of Promega Corporation
Dr. Christopher Eggers received his Ph.D. in biochemistry and molecular biology from the University of California at San Francisco and then completed a postdoctoral fellowship at the Howard Hughes Medical Institute at UC San Diego. Dr. Eggers has been a Senior Research Scientist at Promega since 2011, where he has principally focused on developing bioluminescent assays to measure protein abundance and interactions based on the NanoLuc® and NanoBiT® technologies. This includes leading the technical development of the Nano-Glo® Dual-Luciferase® Reporter Assay and the Nano-Glo® HiBiT Detection Systems.