 |
|
|
|
|
|
|
 |
|
|
| |
- 系統概論
- Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssayTM Kit
- Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Fluorometric BioAssayTM Kit
- Caspase-Glo® Assays
- Apo-ONE® Homogeneous Caspase-3/7 Assay
- CaspACETM Assay System, Fluorometric
- Caspase 3 Apoptosis Detection, Fluorometric BioAssayTM Kit (CPP-32, Apoptain, Yama, SCA-1)
- CaspACETM Assay System, Colorimetric
- Caspase 3 Apoptosis Detection, Colorimetric BioAssayTM Kit (CPP-32, Apoptain, Yama, SCA-1)
- Caspase 3 BioAssayTM ELISA Kit (CPP-32, Apoptain, Yama, SCA-1)
- Caspase 3, Cleaved (Asp175), Apoptosis Marker, IHC Detection Kit, BioAssayTM (CPP-32, Apoptain, Yama, SCA-1)
- Caspase 3, Cleaved (Asp175), Apoptosis Marker, Western Detection Kit (CPP-32, Apoptain, Yama, SCA-1)
- Caspase 6 (McH2), Apoptosis Detection, Colorimetric BioAssayTM Kit
- Caspase 6 (McH2), Apoptosis Detection, Fluorometric BioAssayTM Kit
- Caspase 7 Activity Assay Kit, BioAssayTM (Mch-3, ICE-LAP3, CMH-1)
- Caspase 7 Immunoassay Kit, BioAssayTM (Mch-3, ICE-LAP3, CMH-1)
- Caspase 8 Apoptosis Detection, Colorimetric BioAssayTM Kit
- Caspase 8 Apoptosis Detection, Fluorometric BioAssayTM Kit
- Caspase 8 Staining, Fluorescent In Situ Marker (FLICE, MASH, Mch5)
- Caspase 9, Apoptosis Detection, Fluorometric BioAssayTM Kit (ICE-LAP6, Mch6, Apaf 3)
- Caspase 9 Apoptosis Detection, Colorimetric BioAssayTM Kit
- Caspase 9 Staining, BioAssayTM Kit
- CaspACETM FITC-VAD-FMK In Situ Marker
- Apoptosis Detection, Flow Cytometry BioAssayTM Kit
- Caspase Family Substrate Set, 1,2,3,5,6,8,9 BioAssayTM
- DeadEndTM Fluorometric TUNEL System
- TUNEL Apoptosis Detection Kit (DNA Fragmentation/Fluorescence Staining), BioAssayTM
- DeadEndTM Colorimetric TUNEL System
- Wizard® Genomic DNA Purification Kit
- Apoptosis DNA Ladder Detection BioAssayTM Kit
- Annexin V Apoptosis Detection BioAssayTM Kit
- Apoptosis Assay, Cytochrome C Releasing BioAssayTM Kit
- Mitochondria Cytosol Fractionation BioAssayTM Kit
- Apoptosis Detection, Mitochondria BioAssayTM Kit
- Griess Reagent System
- Nitric Oxide Colorimetric BioAssayTM Kit
- Nitric Oxide Fluorometric BioAssayTM Kit
- Apoptosis Inducer Set, Ready-to-use, BioAssayTM (Actinomycin D, Camptothecin, Cycloheximide, Dexamethasone, Etoposide)
- Caspase Inhibitor Ac-DEVD-CHO
- Caspase Inhibitor Z-VAD-FMK
- Caspase Family Inhibitor Set, BioAssayTM (1,2,3,6,8,9,Family)
- Apoptosis BioAssayTM Sample Kit
- 細胞凋亡相關抗體
系統概論
多數高等真核生物細胞當喪失生理功能或嚴重受損時，會啟動本身的機制自殺，此稱為細胞凋亡(Apoptosis)，或細胞計畫性死亡(Programmed cell death) 。細胞凋亡發生時，染色質濃縮和片段化、細胞質濃縮、細胞收縮、產生細胞質液泡、形成apoptotic bodies等。目前常見的偵測方法，例如Caspase活性分析、片段化DNA偵測、胞膜PS移轉、或分析粒線體的損傷等等。值得注意的是，一般應偵測兩種以上不同的marker以確認細胞凋亡。本公司提供多種細胞凋亡偵測試劑套組，和各式誘導物、抑制劑、反應受質、抗體等等，產品比較請見下表，或可依實驗的樣品種類選擇適當的產品。
表、各種Promega Caspase 3 Assay產品比較
|
|
|
偵測物
|
Caspase 3/7
|
Caspase 3/7
|
Caspase-1 或 3
|
Caspase 3
|
|
反應受質
|
Z-DEVD-aminoluciferin
|
Z-DEVD-R110-DEVD-Z
|
Ac-YVAD-AMC
Ac-DEVD-AMC
|
Ac-DEVD-pNA
|
|
偵測方法
|
冷光
(half-life: 4 hr)
|
螢光
(Ex/Em: 499/521)
|
螢光
(Ex/Em: 360/460)
|
比色
(A405)
|
|
均質試劑
|
是
|
是
|
否
|
否
|
|
反應時間
|
30 分鐘 ~2 小時
|
30 分鐘 ~18 小時
|
1 小時
|
4 小時
|
|
敏銳度
|
★★★★★
|
★★★
|
★★
|
★
|
表、依樣品種類選擇適合的細胞凋亡分析試劑
|
分析標的
|
產品
|
廠牌
|
貨號
|
偵測方法／儀器
|
|
Caspase
|
Caspase-GloTM 2 Assay
|
Promega
|
G0940
|
冷光儀
|
|
Caspase-GloTM 3/7 Assay
|
Promega
|
G8090
|
冷光儀
|
|
Apo-ONE ® Homogeneous Caspase-3/7 Assay
|
Promega
|
G7792
|
螢光儀
|
|
Caspase-GloTM 6 Assay
|
Promega
|
G0970
|
冷光儀
|
|
Caspase-GloTM 8 Assay
|
Promega
|
G8200
|
冷光儀
|
|
Caspase 8 Staining, Fluorescein Active, BioAssayTM Kit (FLICE, MASH, Mch5)
|
USBiological
|
C2087-44
|
流式細胞儀、螢光儀
|
|
Caspase 8 Staining, Red Active, BioAssayTM Kit (FLICE, MASH, Mch5)
|
USBiological
|
C2087-45
|
流式細胞儀、螢光儀
|
|
Caspase-GloTM 9 Assay
|
Promega
|
G8210
|
冷光儀
|
|
Caspase 9 Staining, Fluorescein Active, BioAssayTM Kit
|
USBiological
|
C2088-31
|
流式細胞儀、螢光儀
|
|
Caspase 9 Staining, Fluorescent, Sulforhodamine, BioAssayTM Kit
|
USBiological
|
C2088-32
|
流式細胞儀、螢光儀
|
|
CaspACETM FITC-VAD-FMK In Situ Marker
|
Promega
|
G7461
|
流式細胞儀
|
|
Apoptosis Detection, Flow Cytometry BioAssayTM Kit
|
USBiological
|
A2299-91
|
流式細胞儀
|
|
片段 DNA
|
DeadEndTM Fluorometric TUNEL System
|
Promega
|
G3250
|
螢光顯微鏡
|
|
TUNEL Apoptosis Detection Kit (DNA Fragmentation/Fluorescence Staining), BioAssay TM
|
USBiological
|
T9162-80
|
螢光顯微鏡
|
|
細胞膜 PS 移轉
|
Annexin V (Cy3) Apoptosis Detection BioAssayTM Kit
|
USBiological
|
A2296-30
|
螢光顯微鏡、流式細胞儀
|
|
Annexin V (eGFP) Apoptosis Detection BioAssayTM Kit
|
USBiological
|
A2296-10
|
螢光顯微鏡、流式細胞儀
|
|
Annexin V (FITC) Apoptosis Detection BioAssayTM Kit
|
USBiological
|
A2296-20
|
螢光顯微鏡、流式細胞儀
|
|
粒線體損傷
|
Apoptosis Detection, Mitochondria BioAssayTM Kit
|
USBiological
|
A2299-92
|
螢光顯微鏡、流式細胞儀
|
|
|
|
|
|
|
分析標的
|
產品
|
廠牌
|
貨號
|
偵測方法／儀器
|
|
Caspase-3
|
Anti-ACTIVE ® Caspase-3 pAb
|
Promega
|
G7481
|
顯微鏡
|
|
片段 DNA
|
DeadEndTM Fluorometric TUNEL System
|
Promega
|
G3250
|
螢光顯微鏡
|
|
DeadEndTM Colorimetric TUNEL System
|
Promega
|
G7360
|
光學顯微鏡
|
|
粒線體損傷
|
Anti-Cytochrome c mAb
|
Promega
|
G7421
|
顯微鏡
|
|
PARP
|
Anti-PARP p85 Fragment pAb
|
Promega
|
G7341
|
顯微鏡
|
|
Akt
|
Anti-pS 473 Akt pAb
|
Promega
|
G7441
|
顯微鏡
|
|
分析標的
|
產品
|
廠牌
|
貨號
|
偵測方法／儀器
|
|
Caspase-3
|
Anti-ACTIVE ® Caspase-3 pAb
|
Promega
|
G7481
|
顯微鏡
|
|
Caspase 3, Cleaved (Asp175), Apoptosis Marker, IHC Detection Kit, BioAssayTM (CPP-32, Apoptain, Yama, SCA-1)
|
USBiological
|
C2087-20J
|
顯微鏡
|
|
片段 DNA
|
DeadEndTM Fluorometric TUNEL System
|
Promega
|
G3250
|
螢光顯微鏡
|
|
DeadEndTM Colorimetric TUNEL System
|
Promega
|
G7360
|
光學顯微鏡
|
|
粒線體損傷
|
Anti-Cytochrome c mAb
|
Promega
|
G7421
|
顯微鏡
|
|
PARP
|
Anti-PARP p85 Fragment pAb
|
Promega
|
G7341
|
顯微鏡
|
|
Akt
|
Anti-pS 473 Akt pAb
|
Promega
|
G7441
|
顯微鏡
|
|
分析標的
|
產品
|
廠牌
|
貨號
|
偵測方法／儀器
|
|
Caspase
|
Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Fluorometric BioAssayTM Kit
|
USBiological
|
C2086-75
|
螢光儀
|
|
Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssayTM Kit
|
USBiological
|
C2086-74
|
分光光度計
|
|
CaspACETM Assay System, Fluorometric
|
Promega
|
G3540
|
螢光儀
|
|
Caspase 3 Apoptosis Detection, Fluorometric BioAssayTM Kit (CPP-32, Apoptain, Yama, SCA-1)
|
USBiological
|
C2087-10
|
螢光儀
|
|
CaspACETM Assay System, Colorimetric
|
Promega
|
G7351
|
分光光度計
|
|
Caspase 3 Apoptosis Detection, Colorimetric BioAssayTM Kit (CPP-32, Apoptain, Yama, SCA-1)
|
USBiological
|
C2087-20
|
分光光度計
|
|
Caspase 3 BioAssayTM ELISA Kit (CPP-32, Apoptain, Yama, SCA-1)
|
USBiological
|
C2087-12
|
螢光儀
|
|
Caspase 3, Cleaved (Asp175), Apoptosis Marker,) Western Detection Kit (CPP-32, Apoptain, Yama, SCA-1)
|
USBiological
|
C2087-20K
|
Western Blot
|
|
Caspase 6 (McH2), Apoptosis Detection, Fluorometric BioAssayTM Kit
|
USBiological
|
C2087-26
|
螢光儀
|
|
Caspase 6 (McH2), Apoptosis Detection, Colorimetric BioAssayTM Kit
|
USBiological
|
C2087-25
|
分光光度計
|
|
Caspase 7 Activity Assay Kit, BioAssayTM (Mch-3, ICE-LAP3, CMH-1)
|
USBiological
|
C2087-29P
|
螢光儀
|
|
Caspase 7 Immunoassay Kit, BioAssayTM (Mch-3, ICE-LAP3, CMH-1)
|
USBiological
|
C2087-29B
|
螢光儀
|
|
Caspase 8 Apoptosis Detection, Fluorometric BioAssayTM Kit (FLICE, MASH, Mch5)
|
USBiological
|
C2087-30
|
螢光儀
|
|
Caspase 8 Apoptosis Detection, Colorimetric BioAssayTM Kit
|
USBiological
|
C2087-40
|
分光光度計
|
|
Caspase 9, Apoptosis Detection, Fluorometric BioAssayTM Kit (ICE-LAP6, Mch6, Apaf 3)
|
USBiological
|
C2088-05
|
螢光儀
|
|
Caspase 9 Apoptosis Detection, Colorimetric BioAssayTM Kit
|
USBiological
|
C2088-10
|
分光光度計
|
|
片段 DNA
|
Wizard® Genomic DNA Purification Kit
|
Promega
|
A1120
|
DNA 電泳分析
|
|
Apoptosis DNA Ladder Detection BioAssayTM Kit
|
USBiological
|
A2299-95
|
DNA 電泳分析
|
|
Apoptosis DNA Ladder Detection (Enhanced) BioAssayTM Kit
|
USBiological
|
A2299-96
|
DNA 電泳分析
|
|
粒線體損傷
|
Apoptosis Assay, Cytochrome C Releasing BioAssayTM Kit
|
USBiological
|
A2299-90
|
Western Blot
|
|
PARP
|
Anti-PARP p85 Fragment pAb
|
Promega
|
G7341
|
Western blot
|
|
Akt
|
Anti-pS 473 Akt pAb
|
Promega
|
G7441
|
Western blot
|
|
 |
|
 |
|
|
|
|
 |
