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試劑 > Cell > Signal Transduction and Drug Screening
 

系統概論

Promega藥物篩選系統特色
 1.簡單、精確、靈敏
 2.Ultra high-throughput
 3.Primary screening and secondary screening
 4.Bioluminescent assay
   
 
傲視群雄的生物冷光科技:專利Ultra-GloTM Luciferase更亮、更穩定
   
Promega藥物篩選系統特性比較

品名

偵測訊號

分析類別

自動化

規格

Add-Mix-Read

GloSensorTM cAMPAssay

冷光

Live-Cell Assay

Yes

96, 384, 1536

Yes

cAMP-Glo Assay

冷光

Lytic assay

Yes

96, 384, 1536

Yes

PDE-GloTM Assay

冷光

Enzyme based assay

Yes

96, 384, 1536

Yes

品名

偵測訊號

Robustness (Z)

自動化

規格

Add-Mix-Read

Bright-GloTM Luciferase Assay System

冷光

++++

Yes

96, 384, 1536

Yes

Steady-Glo® Luciferase Assay System

冷光

+++

Yes

96, 384, 1536

Yes

Dual-GloTM Luciferase Assay System

冷光

+++++

Yes

96, 384, 1536

Yes

EnduRenTM Live Cell Substrate

冷光

+++

Yes

96, 384, 1536

Live cell

品名

偵測訊號

Robustness (Z)

靈敏度

規格

Add-Mix-Read

CellTiter-Glo® Luminescent Cell Viability Assay

冷光

++++

<10 cells

96, 384, 1536

Yes

MultiTox-Fluor Multiplex Cytotoxicity Assay

螢光

++++

10–20

96, 384

Yes

CellTiter-Blue® Cell Viability Assay

螢光

+++

50

96, 384, 1536

Yes

CellTiter 96® AQueous One Solution Cell Proliferation Assay

比色

+++

200

96, 384

Yes

CytoTox-ONETM Homogeneous Membrane Integrity Assay

螢光

+++

200

96, 384, 1536

Yes

Apo-ONE® Homogeneous Caspase-3/7 Assay

螢光

+++

200

96, 384

Yes

Caspase-Glo® 3/7 Assay

冷光

++++

20

96, 384

Yes

Caspase-Glo® 8 Assay

冷光

++++

~1,000

96, 384

Yes

Caspase-Glo® 9 Assay

冷光

++++

~1,500

96, 384

Yes

品名

偵測訊號

靈敏度

自動化

規格

Add-Mix-Read

Protease-GloTM Assay

冷光

ND

ND

96, 384, 1536

Yes

Calpain-GloTM Protease Assay

冷光

5pM enzyme

Yes

96, 384

Yes

DPPIV-GloTM Protease Assay

冷光

1pg/ml enzyme

Yes

96, 384

Yes

Proteasome-GloTM Cell-Based Assay

冷光

100 cells

Yes

96, 384, 1536

Yes

Proteasome-GloTM Chymotrypsin-Like Assay

冷光

0.5ng/ml enzyme

Yes

96, 384, 1536

Yes

Proteasome-GloTM Trypsin-Like Assay

冷光

0.5ng/ml enzyme

Yes

96, 384, 1536

Yes

Proteasome-GloTM Caspase-Like Assay

冷光

0.5ng/ml enzyme

Yes

96, 384, 1536

Yes

品名

偵測訊號

Robustness (Z)

自動化

規格

Add-Mix-Read

Kinase-Glo® and Kinase-Glo® Plus Luminescent Kinase Assays

冷光

+++++

Yes

96, 384, 1536

Yes

ProFluor® Kinase Assays: PKA, Tyrosine Phosphatase, Src-Family

螢光

+++++

Yes

96, 384

Yes

SignaTECT® Kinase Assays: PKA, PKC, PTK, CaM KII, cdc2, DNA-PK

放射性

++++

No

96

No

SAM2® Biotin Capture Membrane and Plate

放射性

++++

Yes

96 and higher

No

品名

偵測訊號

Robustness (Z)

自動化

規格

Add-Mix-Read

P450-GloTM Screening Systems:

CYP1A2, CYP2C9, CYP3A4, CYP2C19, CYP2D6

冷光

+++++

Yes

96, 384, 1536

Yes

P450-GloTM Assays:

CYP1A1, CYP1B1, CYP1A2, CYP2C8, CYP2C9, CYP3A4, CYP3A7, CYP2C19, CYP2D6

冷光

+++++

Yes

96, 384, 1536

Yes

MAO-GloTM Assay

冷光

+++++

Yes

96, 384

Yes

Pgp-GloTM Assay System with Pgp

冷光

+++++

Yes

96, 384

Yes

See www.promega.com/automethods/ for a current listing of automatable methods using the following instrumentation: Aurora Discovery, Beckman Coulter, Caliper Life Sciences, Deerac Fluidics, Eppendorf, Perkin Elmer, Tecan and Thermo Electron.

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