系統概論

體外蛋白表現（Cell-free Protein expression system）系統粗分為下列兩種型式：

RNA-based
DNA-based (circular, linear and PCR product)

RNA-based的樣本包含RNA、mRNA或體外合成的轉錄片段；而NA-based為質體或含有位於轉錄啟動子和轉譯起始點的基因（例如PCR/RTPCR產物）。選擇適當的蛋白表現系統需考量幾個因素，其中包括基因的來源(真核/原核)、起始樣本類型(RNA或DNA)以及蛋白的後續應用等等，不一而足。

應用
 

>	Protein:protein interaction.
>	Protein:nucleic acid interactions
>	Protein:ligand interaction.
>	Enzyme assays.
>	Protein expression.

 

DNA based cell-free expression
systems
 

1Only T7 linear templates.
2T7 promoter only, must use provided T7 TNTR PCR Enhancer.
3Must be linearized for T7.
4 SP6 and T3 promoters only.

mRNA-Driven Translation Systems
 

產品手冊
 

CELL-FREE PROTEIN EXPRESSION, EUKARYOTIC SYSTEMS		PDF (size)
TM305	TNT® T7 Insect Cell Extract System	320kb
TM282	TNT® SP6 High-Yield Protein Expression System	311kb
TM045	TNT® Quick Coupled Transcription/Translation System	421kb
TB126	TNT® Coupled Reticulocyte Lysate System	435kb
TB165	TNT® Coupled Wheat Germ Extract Systems	408kb
TM232	Rabbit Reticulocyte Lysate System	302kb
TM235	TNT® T7 Quick for PCR DNA	209kb
TB127	Flexi® Rabbit Reticulocyte Lysate System	351kb
TM230	Wheat Germ Extract System	276kb
TM066	Wheat Germ Extract Plus	244kb

 

CELL-FREE PROTEIN EXPRESSION, PROKARYOTIC SYSTEMS		PDF (size)
TB219	E. coli T7 S30 Extract for Circular DNA	301kb
TB092	E. coli S30 Extract System for Circular DNA	278kb
TB102	E. coli S30 Extract System for Linear Templates	299kb
TM306	S30 T7 High-Yield Protein Expression System	368kb
TB293	T7 Sample System	163kb
TM282	TNT® SP6 High-Yield Wheat Germ Protein Expression System	311kb